2021 State Qualifying Time Standard: :59.79
| 1 |  Grace Frericks, SRCedar Falls | 55.91 |
| 2 |  Meghan Donald, SRAmes | 56.04 |
| 3 |  Scarlet Martin, SRIowa City West | 56.56 |
| 4 |  Hannah Cousins, SODavenport Central | 57.96 |
| 5 |  Olivia Swalley, JRJohnston | 58.38 |
| 6 |  Alexis Henning, SRAnkeny | 58.50 |
| 7 |  Jenna Currier, SRCarroll | 58.58 |
| 8 | Jordon Kloth, SR Ames | 58.68 |
| 9 |  Payden Rafferty, SOWaukee | 58.73 |
| 10 | Abby Hala, SR Ames | 58.92 |
| 11 | Evan Schwickerath, JR Waukee | 58.96 |
| 12 |  Julia Sweetman, JRDowling Catholic | 59.49 |
| 13 | Carlee Wilkins, SO Iowa City West | 59.53 |
| 14 | Jessi Wigham, JR Waukee | 59.56 |
| 15 | Zoe Davey, FR Waukee | 59.64 |
| 16 | Abby Wilcox, SO Dowling Catholic | 59.73 |